A method for determining urinary concentrations of 2,4-D in samples co
llected from non-occupationally, environmentally exposed individuals w
as developed. The 2,4-D was extracted from fortified human urine sampl
es using octadecylsilane solid phase extraction cartridges. The averag
e percent recovery for urine samples spiked at 2 and 20 ng/mL was 100%
and 93%, respectively. The method detection limit was estimated to be
0.75 ng of 2,4-D per mL of urine based on a 10 mL sample size. The po
tential use of 2,4-dichlorophenylacetic acid as a surrogate standard w
as also investigated. Copyright (C) 1996 Elsevier Science Ltd.