DIFFERENTIAL EXPRESSION OF PSORIASIN MESSENGER-RNA BETWEEN IN-SITU AND INVASIVE HUMAN BREAST-CARCINOMA

The mRNA encoding the calcium-binding protein psoriasin was identified as bring more highly expressed in the in situ versus the invasive com ponent of the same breast tumor. Reverse transcription-PCR analysis of total RNA extracted from three independent cases of ductal carcinoma in situ of the comedo type and three cases of high-grade invasive duct al carcinoma revealed a detectable level of psoriasin mRNA expression in the iia situ lesions only. Similar analysis performed on total RNA extracted from frozen sections of 32 independent breast samples, repre senting a continuum from normal to invasive tumor, confirmed high psor iasin expression in ductal carcinoma in situ of the comedo type only. The possible functional role of the psoriasin protein in breast tumor cells remains to be determined.