DNA-SEQUENCE ANALYSIS OF A 10,624 BP FRAGMENT OF THE LEFT ARM OF CHROMOSOME-XV FROM SACCHAROMYCES-CEREVISIAE REVEALS A RNA-BINDING PROTEIN,A MITOCHONDRIAL PROTEIN, 2 RIBOSOMAL-PROTEINS AND 2 NEW OPEN READING FRAMES

We have determined the sequence of a 10 624 bp DNA segment located in the left arm of chromosome XV of Saccharomyces cerevisiae. The sequenc e contains eight open reading frames (ORFs) longer than 100 amino acid s. Two of them do not present significant homology with sequences foun d in the databases. The product of ORF o0553 is identical to the prote in encoded by the gene SMF1. Internal to it there is another ORF, o055 5 that is apparently expressed. The proteins encoded by ORFs o0559 and o0565 are identical to ribosomal proteins S19.e and L18 respectively. ORF o0550 encodes a protein with an RNA binding signature including R NP motifs and stretches rich in asparagine, glutamine and arginine. Th e nucleotide sequence determined has been deposited in the EMBL data l ibrary under Accession Number X95258.