DNA-SEQUENCE ANALYSIS OF THE VPH1-SNF2 REGION ON CHROMOSOME-XV OF SACCHAROMYCES-CEREVISIAE

The nucleotide sequence of a 37 000 base pair region from the left arm of chromosome XV of Saccharomyces cerevisiae has been determined and analysed. This region contains 21 open reading frames (ORFs) coding fo r proteins of more than 100 amino acids. Six ORFs correspond to the ge nes PA CI, VPH1, MOD5. CAP20, ORF1 and SNF2 already described. Eight O RFs show some similarities to known genes from yeast and other organis ms. They include genes coding for serine/threonine protein kinases, a multidrug resistance family homologue, a protein related to dihydrofol ate reductase, a cluster of heat shock-like proteins and a gene coding for an enzyme related to protein disulfide isomerase. Finally seven O RFs do not show any similarities with a known gene. In addition we fou nd a new ala-tRNA (UGC) gene located next to a sigma sequence. The seq uence has been deposited in the EMBL databank under Accession Number X 89633.