THE SEQUENCE OF 23 KB SURROUNDING THE SNF3 LOCUS ON THE LEFT ARM OF YEAST CHROMOSOME-IV REVEALS THE LOCATION OF 5 KNOWN GENES AND CHARACTERIZES AT LEAST 6 NEW OPEN READING FRAMES INCLUDING PUTATIVE GENES FOR RIBOSOMAL-PROTEIN L35 AND A SUGAR-TRANSPORT PROTEIN

The nucleotide sequence of 22,846 bp of the left arm of chromosome IV is described. Twelve open reading frames (ORFs) greater than 100 tripl ets were detected, one of which extends into an adjacent cosmid. Two o f the ORFs may contain an intron. One of these is an L35 ribosomal pro tein gene. Five ORFs (D1204, D1214, D1219, D1234 and D1244) encode pre viously sequenced genes (MGT1, SHM1, ASF2, SNF3 and ARF2, respectively ). The nucleotide sequence of a sixth ORF (D1229) is quite similar to the WEB1 gene, which appeared in the DNA databases shortly after finis hing the sequence reported here. It is not clear whether or not WEB1 a nd D1229 represent one and the same gene. The co-linearity of the repo rted DNA sequences with the genome of strains from Saccharomyces cerev isiae subspecies carlsbergensis, sake and diastaticus was assessed by comparative PCR with overlapping primer sets. The nucleotide sequence data reported in this paper will appear in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under Accession Number X83276.