THE DNA-SEQUENCE OF COSMID-14-5 FROM CHROMOSOME-XIV REVEALS 21 OPEN READING FRAMES INCLUDING A NOVEL GENE ENCODING A GLOBIN-LIKE DOMAIN

In this paper is described the DNA sequence of cosmid 14-5 from chromo some XIV of Saccharomyces cerevisiae. The sequence is 38 855 bases lon g and contains 21 open reading frames (ORFs) plus three internal ORFs. Six ORFs correspond to known yeast genes (SLA2, ZWF1, BLH1, KEX2, SIN 4 and URE2); two other ORFs had already been sequenced because they ar e adjacent to known genes; the remaining 12 ORFs are novel genes. Of t hese, one ORF (N1142) is particularly interesting since it shows a sig nificant similarity to mammalian globin. Another ORF (N1254) displays two zinc finger motifs as well as a DNAJ motif. The cosmid sequence ha s been submitted to the EMBL data library under Accession Number Z6938 1.