NUCLEOSIDE 5'-TRIPHOSPHATES MODIFIED IN THE SUGAR MOIETY AS SUBSTRATES FOR DNA-POLYMERASES

Citation
Lp. Savochkina et al., NUCLEOSIDE 5'-TRIPHOSPHATES MODIFIED IN THE SUGAR MOIETY AS SUBSTRATES FOR DNA-POLYMERASES, Collection of Czechoslovak Chemical Communications, 61, 1996, pp. 63-67
Citations number
16
Categorie Soggetti
Chemistry
ISSN journal
00100765
Volume
61
Year of publication
1996
Pages
63 - 67
Database
ISI
SICI code
0010-0765(1996)61:<63:N5MITS>2.0.ZU;2-6
Abstract
Substrate properties of new nucleoside 5'-triphosphates modified in th e sugar moiety 1c-5c toward E. coli DNA polymerase I, Klenow fragment, reverse transcriptases of HIV-I, AMV and M-MLV, and DNA polymerase fr om Thermus aquaticus were investigated. The latter enzyme showed stric t substrate specificity, being inactive toward all of the 5'-triphosph ates tested. Among the analogues assayed, the best termination substra te properties were displayed by triphosphate 5c, while compounds 1c-3c were intermediate in activity and analogue 4c was much less effective .