SYNTHESIS AND CHARACTERISTICS OF A SELF-COMPLEMENTARY TETRAMER D(CGCG) WITH METHYLPHOSPHONATE LINKAGES OF DEFINED STEREOCHEMISTRY

Authors
SCHWEITZER M SAMSTAG W ENGELS JW
Citation
M. Schweitzer et al., SYNTHESIS AND CHARACTERISTICS OF A SELF-COMPLEMENTARY TETRAMER D(CGCG) WITH METHYLPHOSPHONATE LINKAGES OF DEFINED STEREOCHEMISTRY, Collection of Czechoslovak Chemical Communications, 61, 1996, pp. 213-216
Citations number
11
Categorie Soggetti
Chemistry
Journal title
Collection of Czechoslovak Chemical Communications → ACNP
ISSN journal
00100765
Volume
61
Year of publication
1996
Pages
213 - 216
Database
ISI
SICI code
0010-0765(1996)61:<213:SACOAS>2.0.ZU;2-I
Abstract
Starting from protected deoxynucleosides CpG methylphosphonate dimers (p indicating methylphosphonate linkage) were prepared and separated i nto diastereomers by flash chromatography. The Rp isomer was converted into the beta-cyanoethyl phosphoramidite and coupled in solution with 5'-deprotected CpG to form a self-complementary tetramer d(CpGCpG) wi th two Rp configurated methylphosphonate moieties. This molecule is us ed in thermodynamic studies of the duplex to coil, and the B to Z tran sition and its structure is investigated by 2D NMR methods.