SYNTHETIC OLIGONUCLEOTIDE COMBINATORIAL LIBRARIES WITH SINGLE BEAD SEQUENCE IDENTIFICATION

Authors
ROTTE B HINZ M BADER R ASTRIAB A MARKIEWICZ M MARKIEWICZ WT SELIGER H
Citation
B. Rotte et al., SYNTHETIC OLIGONUCLEOTIDE COMBINATORIAL LIBRARIES WITH SINGLE BEAD SEQUENCE IDENTIFICATION, Collection of Czechoslovak Chemical Communications, 61, 1996, pp. 304-306
Citations number
6
Categorie Soggetti
Chemistry
Journal title
Collection of Czechoslovak Chemical Communications → ACNP
ISSN journal
00100765
Volume
61
Year of publication
1996
Pages
304 - 306
Database
ISI
SICI code
0010-0765(1996)61:<304:SOCLWS>2.0.ZU;2-1
Abstract
Several types of beads have been used to prepare oligonucleotide libra ries and their molecular recognition properties tested through hybridi zation screening. Polyoxyethylene-grafted polystyrene beads (Tentagel( R), Rapp Polymers) were found to have good library properties and. in addition. allowed the identification of the immobilized sequence by di rect Maxam-Gilbert analysis of the oligcnucleotides released from a si ngle bead.