DEVELOPMENT OF A RAPID DETECTION PROCEDURE FOR CRYPTOSPORDIUM, USING IN-VITRO CELL-CULTURE COMBINED WITH PCR

Authors
ROCHELLE PA FERGUSON DM HANDOJO TJ DELEON R STEWART MH WOLFE RL
Citation
Pa. Rochelle et al., DEVELOPMENT OF A RAPID DETECTION PROCEDURE FOR CRYPTOSPORDIUM, USING IN-VITRO CELL-CULTURE COMBINED WITH PCR, The Journal of eukaryotic microbiology, 43(5), 1996, pp. 72-72
Citations number
7
Categorie Soggetti
Zoology,Microbiology
Journal title
The Journal of eukaryotic microbiology → ACNP
ISSN journal
10665234
Volume
43
Issue
5
Year of publication
1996
Pages
72 - 72
Database
ISI
SICI code
1066-5234(1996)43:5<72:DOARDP>2.0.ZU;2-K
Abstract
A detection, viability, and infectivity assay was developed for Crypto sporidium parvum. Oocysts or excysted sporozoites were inoculated onto monolayers of CaCo-2 cells grown on chamber slides. C. parvum infecti on was monitored by three methods: a) application of a fluorescein-lab eled anti-sporozoite antibody, b) PCR of a heat-shock protein gene fra gment, and c) detection of mRNA from the heat-shack protein gene by RT -PCR.