AUTOMATED ANALYTICAL SYSTEMS FOR DRUG DEVELOPMENT STUDIES .5. A SYSTEM FOR ENZYME-KINETIC STUDIES

Two similar automated analytical systems using liquid chromatography ( LC) and microdialysis as an on-line sampling technique were applied to studies of enzyme kinetics. 2',3',5'-Triacetyl-6-azauridine (azaribin e) with porcine liver esterase (PLE) and N-acetylphenylalanyl-3,5-diio dotyrosine (AcFY') with pepsin were used as model compounds. The micro dialysis sampling technique permitted the rapid separation of low mole cular weight analytes from macromolecules, thus simultaneously achievi ng clean-up of the samples and quenching of the reaction. The combinat ion of rapid LC analysis and microdialysis sampling provided selectivi ty and automation. The systems are rugged and give reproducible result s in agreement with those from manual sampling methods.