INVESTIGATION OF NEWLY SYNTHESIZED ENDOTHELIN PEPTIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COUPLED TO ELECTROSPRAY MASS-SPECTROMETRY

Authors
ROVATTI L CURCURUTO O HAMDAN M CASSANO E GALOPPINI C ROVERO P
Citation
L. Rovatti et al., INVESTIGATION OF NEWLY SYNTHESIZED ENDOTHELIN PEPTIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COUPLED TO ELECTROSPRAY MASS-SPECTROMETRY, Rapid communications in mass spectrometry, 10(12), 1996, pp. 1504-1508
Citations number
11
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
Journal title
Rapid communications in mass spectrometry → ACNP
ISSN journal
09514198
Volume
10
Issue
12
Year of publication
1996
Pages
1504 - 1508
Database
ISI
SICI code
0951-4198(1996)10:12<1504:IONSEP>2.0.ZU;2-O
Abstract
A number of newly synthesized endothelin peptides have been examined b y high performance liquid chromatography/electrospray mass spectrometr y (HPLC/ES-MS). The presented data allowed reliable identification of both the target peptides and a number of associated side products incl uding the target peptide in methylated form and peptide sequences with relative molecular masses lower than those desired. Characteristic fr agments in the ES mass spectra permitted differentiation between the m ethylation sites involving the carboxylic functions of the C-terminus and the side-chain of the aspartic acid.