SOLVENT SYSTEMS IN CENTRIFUGAL PARTITION CHROMATOGRAPHY

Centrifugal partition chromatography (CPC) or countercurrent chromatog raphy (CCC) is a kind of liquid-liquid chromatography without solid ph ase. Two immiscible liquids in equilibrium share the total volume of a ''column'' designed to keep one phase pseudo-stationary. A tremendous variety of biphasic systems are available, which can lead to some dif ficulty when a selection must be made. Ternary diagrams, describing mi xtures of three solvents, and systems with four solvents or more, used by research scientists familiar with this chromatographic technique, are useful guides for a rational choosing of a biphasic system in CCC & CPC.