DETERMINATION OF GLUTATHIONE-PEROXIDASE KINETIC-PARAMETERS BY EXPERIMENTAL-DESIGN

Experimental design methodology permits to determine experimental cond itions in order to maximize the enzymatic initial rate reaction. After determination of factors of the study, we have proposed a model by us ing a Doehlert uniform shell design. The study of the response equatio n and the canonical equation has given factor Values that optimize the experimental response which is the enzymatic initial rate reaction. F rom the obtained mathematical model, we have thus been able to determi ne the critical factors as well as Michaelis-Menten constants and Dalz iel coefficients to specify the kinetic mechanism without supplementar y manipulations. We have chosen to apply the experimental design by st udying the kinetics of a well known enzyme: the glutathione peroxidase .