DETERMINATION OF THE ALKALOIDS IN EVODIAE-FRUCTUS BY CAPILLARY ELECTROPHORESIS

A total of ten Evodia alkaloids was separated successfully by micellar electrokinetic capillary chromatography (MECC) and capillary zone ele ctrophoresis (CZE). The MECC method based on SDS was applied to analyz e three indolequinazoline alkaloids (evodiamine, rutaecarpine and carb oxyevodiamine) and six quinolone alkaloids (1-methyl-2-nonyl-4(1H)-qui nolone, 1-methyl-2-[(Z)-6- undecenyl] -4(1H)-quinolone, 1-methyl-2-und ecyl-4(1H)-quinolone, evocarpine, 1-methyl-2-[(6Z,9Z)-6,9-pentadecadie nyl]-4( 1H)-quinolone and dihydroevodiamine) in 15 min and CZE techniq ue was used to determine the dehydroevodiamine in 5 min. Linearity ove r one order of magnitude of concentration was generally obtained and l imits of detection for the alkaloids were in the range of 35-47 mu g/m l. The relative standard deviations were less than 4% (n=6). Contents of Evodiae alkaloids in a methanol-water extract of Evodia fructus sam ple could easily be determined by this method. The effects of pH, surf actant concentration and organic modifier concentration of the carrier on the migration behaviour of the solutes are discussed.