LOCALIZATION OF NITRIC-OXIDE SYNTHASE-I AND SYNTHASE-III IN THE COCHLEA

Nitric oxide synthase (NOS) isoforms I and III were localized in the g uinea pig cochlea by indirect immunohistochemistry using frozen sectio ns and paraffin sections. NOS I staining was observed in the cytoplasm of outer hair cells, in nerve cell somata and fibers of the spiral ga nglion, and in axonal profiles of the spiral lamina next to the base o f inner hair cells. In addition, lining cells of the inner sulcus and limbus, and cells of the spiral ligament stained for NOS I but vascula r walls remained unstained. NOS III reactivity was seen in the cytopla sm of outer and inner hair cell, in lining cells of the limbus, and on the endolymphatic surface of marginal cells. Staining for NOS III of spiral ganglion perikarya showed varying intensity. Endothelial cells of cochlear glomeruli reacted for NOS III. NOS III in vascular endothe lial cells implies regulatory effects of nitric oxide (NO) on vascular wall tonus and cochlear blood supply. NOS I in cochlear neurons indic ates these cells as possible sources for NO during neuronal activity. Activated neurons may provide NO that adjusts cochlear perfusion to ne uronal activity. Finally, NO that is liberated from hair cells or affe rent synaptic terminals may act as an inhibitor on N-methyl-D-aspartat e (NMDA) receptors (negative feed-back inhibition).