GLUCOSE-OXIDASE AND CATALASE ACTIVITIES OF PENICILLIUM VARIABILE P16 IMMOBILIZED IN POLYURETHANE SPONGE

Conidia of Penicillium variabile P16 were immobilized in polyurethane sponge and used in repeated-batch processes in a fluidized-bed reactor . Optimal conditions for production of glucose oxidase and catalase we re: inoculum size, 10%; glucose concentration, 80 g L(-1); Ca-carbonat e concentration, 15 g L(-1); temperature, 28 degrees C and aeration ra te, 4 V V-1 min(-1). In an extended repeated-batch process, glucose ox idase activity was highest after the fourth batch and catalase activit y was highest after the fifth batch. Scanning electron microscopy show ed that the fungus grew only in the interior of carrier particles.