ISCHEMIA-REPERFUSION INJURY IN THE SPINAL-CORD OF RABBITS STRONGLY ENHANCES LIPID-PEROXIDATION AND MODIFIES PHOSPHOLIPID PROFILES

The effect of spinal cord ischemia (10, 20, and 40 min) and post-ische mic reperfusion (10, 30, and 60 min) on lipid peroxidation and phospho lipids was investigated. Spinal cord ischemia was accompanied by lipol ytic processes with significant changes in concentration of lipid pero xidation products (LPP). Reestablishment of the blood supply after 10 min ischemia was accompanied by significantly increased levels of thio barbituric acid reactive substances (TBA-RS) after 10 and 30 min of re perfusion. Following 20 and 40 min ischemia a significant increase was observed at all reperfusion periods. Ischemia itself significantly re duced the concentration of phosphatidyl inositol (IP), phosphatidyl et hanolamine (EP) and ethanolamine plasmalogens (Epls). Significant chan ges were observed in concentration of phosphatidyl serine (SP) too, bu t only after 20 and 40 min of ischemia. The concentration of phosphati dic acid (PA) was significantly reduced only after 10 min of ischemia The onset of reperfusion after ischemia was accompanied by a diverse p attern of changes in PA, IP, Epls and SP, while the concentration of E P remained at the above mentioned ischemic intervals.