Dd. Songstad et al., PRODUCTION OF TRANSGENIC MAIZE PLANTS AND PROGENY BY BOMBARDMENT OF HI-II IMMATURE EMBRYOS, In vitro cellular & developmental biology. Plant, 32(3), 1996, pp. 179-183
Production of transgenic maize (Zea mays L.) callus, plants, and proge
ny from microprojectile bombardment of 2-5-d cultured Hi-II immature e
mbryos is described. Histological evidence indicates that these tissue
s are amenable to transformation due to surface layer cell division of
the scutellum. Two out of every 100 bombarded embryos produced transg
enic callus and R(0) transgenic plants were both male and female ferti
le. Expected segregation of transgenes was observed in progeny. The pr
imary advantage of bombarding these tissues is increased male and fema
le fertility of transgenic plants compared with those produced using l
ong-term callus or suspension cultures.