THE METASTASIS SUPPRESSOR CANDIDATE NUCLEOTIDE DIPHOSPHATE KINASE NM23 SPECIFICALLY INTERACTS WITH MEMBERS OF THE ROR RZR NUCLEAR ORPHAN RECEPTOR SUBFAMILY/

We have cloned proteins that interact with the nuclear orphan receptor RZR beta using the yeast two-hybrid system. We identified, amongst a number of ether genes, the nucleoside diphosphate kinase (NDPK)-2, als o known as Nm23-2, c-myc regulator; factor PuF and differentiation inh ibitory factor. RZR beta specifically interacts with Nm23-2 but not wi th he closely related tumor metastasis suppressor candidate gene produ ct Nm23-1. In contrast, ROR alpha interacts with both Nm23 proteins. T hese findings were corroborated by ill vitro interaction assays based on GST-pulldown experiments. With n-myc we propose a candidate gene re gulated by ROR alpha/RZR beta and Nm23, based on the Finding that the respective DNA binding sites in the first intron are conserved in seve ral mammalian species. (C) 1996 Academic Press, Inc.