Gr. Vanderhoff et al., DETERMINATION OF ORGANOCHLORINE COMPOUNDS IN FATTY MATRICES - APPLICATION OF RAPID OFF-LINE NORMAL-PHASE LIQUID-CHROMATOGRAPHIC CLEANUP, Journal of chromatography, 754(1-2), 1996, pp. 487-496
Citations number
17
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A clean-up method for organochlorine compounds in fatty samples based
on normal-phase liquid chromatography (NPLC) is described. To this end
, an existing clean-up procedure which uses column switching for the s
eparation of organochlorine pesticides (OCPs) and polychlorinated biph
enyls (PCBs) from the fat matrix was simplified to a single silica LC
column procedure. The use of an LC column packed with 3 mu m silica en
ables complete fat/OCP separation in a total fraction volume of 12 ml,
and results in a fully automated clean-up procedure that takes only 3
2 min per sample. The method showed average recoveries of 80-110% in t
he concentration range of 1-510 mu g/kg, with relative standard deviat
ions of less than 10%. Limits of determination were in the range of 0.
5-50 mu g/kg. The simplified approach has shown its potential for a va
riety of samples, such as milk, pork fat, animal feed and cod liver oi
l, showing its general applicability to fatty samples.