Y. Wang et al., A PROPOSAL TO TRANSFER MICROBISPORA-BISPORA (LECHEVALIER 1965) TO A NEW GENUS, THERMOBISPORA GEN-NOV, AS THERMOBISPORA-BISPORA COMB-NOV, International journal of systematic bacteriology, 46(4), 1996, pp. 933-938
We determined almost complete 16S rRNA gene sequences of two Microbisp
ora bispora (Lechevalier 1965) strains, ATCC 19993(T) (T = type strain
) and JCM 3082, The two sequences were 99% similar to each other but e
xhibited only 81 to 87.8% similarity with the 16S rRNA gene sequences
of seven other Microbispora strains, A phylogenetic analysis revealed
that the two sequences clustered not only distantly from other Microbi
spora strains, but also outside the cluster containing members of the
family Streptosporangiaceae. On the basis of the results of our phylog
enetic analysis and the results of a comprehensive review of the genus
Microbispora by Miyadoh et al. (S. Miyadoh, S. Amano, H. Tohyama, and
T. Shomura, J. Gen, Microbiol, 136:1905-1913, 1990) in which chemotax
onomic and DNA-DNA hybridization analyses were performed, we propose t
hat Microbispora bispora should be transferred to a new genus, Thermob
ispora gen, nov., as Thermobispora bispora comb, nov.