A NOVEL STOPPED-FLOW METHOD FOR MEASURING THE AFFINITY OF ACTIN FOR MYOSIN HEAD FRAGMENTS USING MU-G QUANTITIES OF PROTEIN

The dissociation constant for actin binding to myosin and its subfragm ents (S1 & HMM) is much less than 1 mu M at physiological ionic streng th. Many of the methods used to measure such affinities are unreliable for a K-d below 0.1 mu M. We show here that the use of phalloidin to stablise F-actin and fluorescently labelled proteins allows the affini ty of actin for myosin S1 to be measured in a simple transient kinetic assay. The method can be used for K-d's as low as 10 nM and we demons trate that the K-d's can be estimated using only mu g quantities of ma terial. Furthermore we suggest how this method may be adapted for ng q uantities of protein. This will allow the affinity of actin for myosin fragments to be estimated for proteins which are difficult to obtain in large quantities i.e. from biopsy material or from proteins express ed in baculovirus.