BIOLOGICAL-ACTIVITY OF RECOMBINANT HUMAN GROWTH-HORMONE AND ITS MUTANTS

Authors
BELOUSOVA YV SHULGA AA GABRIELYAN AE LEVICHKIN IV LOPATIN SA CHERNOV BK ZAKHAROVA IV KUZINA NV MIKHAILOVA LI MOSKALEVA EY KONDRATENKO TY SEVERIN ES SKRYABIN KG KIRPICHNIKOV MP
Citation
Yv. Belousova et al., BIOLOGICAL-ACTIVITY OF RECOMBINANT HUMAN GROWTH-HORMONE AND ITS MUTANTS, Molecular biology, 30(3), 1996, pp. 401-405
Citations number
26
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
30
Issue
3
Year of publication
1996
Part
2
Pages
401 - 405
Database
ISI
SICI code
0026-8933(1996)30:3<401:BORHGA>2.0.ZU;2-H
Abstract
Hybrid molecules of human growth hormone (GH) and porcine GH were cons tructed using site-directed mutagenesis to obtain human GH mutants wit h multiple (R8S, D11A, M14V, H18Q, R19H), (Y164S, R167K), (Y164S, R167 K, D171H) or single Y35S, R64A, or Q181 amino acid substitutions. When tested with the Nb2 lymphoma cells, these substitutions had almost no effect on the GH growth-promoting activity. However, GH binding to ce ll receptors was altered.