M. Letellier et al., PERFORMANCE EVALUATION OF AUTOMATED IMMUNOASSAYS ON THE TECHNICON-IMMUNE-1 SYSTEM, Clinical chemistry, 42(10), 1996, pp. 1695-1701
We performed an immunoassay evaluation for various analytes on a fully
automated random-access analyzer, the Technicon Immune 1(R) system fr
om Bayer Corp. This system involves latex agglutination, magnetic sepa
ration sandwich, and magnetic separation competitive immunoassay confi
gurations. The evaluated analytes were thyrotropinn (TSH), triiodothyr
onine, thyroxine, free thyroxine, follitropin, lutropin, prolactin, be
ta subunit of human chorionic gonadotropin, cortisol, ferritin, alpha-
fetoprotein, carcinoembryonic antigen, and prostate-specific antigen.
We tested the assay precision, linearity, and correlation with compari
son methods for these analytes. The functional sensitivity of the TSH
assay and the sample-to-sample carryover were also studied. Excellent
results were obtained for within-run and between-day precision studies
, with most assays showing within-run CVs < 4% and between-day CVs < 6
%. The linearity for all assays was acceptable and the correlation bet
ween Immune 1 assays and comparison methods showed satisfactory result
s. The functional sensitivity of the TSH assay was estimated at 0.04 m
U/L. No sample-to-sample carryover was detected.