Je. Wellington et al., THE HIGHLY O-GLYCOSYLATED GLYCOPROTEIN GP2 OF EQUINE HERPESVIRUS-1 ISENCODED BY GENE-71, Journal of virology, 70(11), 1996, pp. 8195-8198
There have been conflicting reports regarding the gene assignment of t
he high-molecular-mass envelope glycoprotein gp2 (gp300) of equine her
pesvirus 1. Here, we provide an unequivocal demonstration that gp2 is
encoded by gene 71. gp2 that was purified with a defining monoclonal a
ntibody was cleaved internally to yield a 42-kDa protein encoded by ge
ne 71. Amino acid composition data and N-terminal sequence analysis of
a tryptic peptide identified gp2 as the product of equine herpesvirus
1 gene 71 with the SWISS-PROT database. Analysis of gp2's monosacchar
ide composition and the 42-kDa subunit showed that the high level of O
glycosylation occurs on the serine/threonine-rich region upstream of
the cleavage site.