INTERSTITIAL FLUID APOLIPOPROTEIN A-II - AN ASSOCIATION WITH THE OCCURRENCE OF MYOCARDIAL-INFARCTION

A sample of male patients aged 25-64 years, survivors of myocardial in farction (MI) taken from the Lille MONICA register, and age-matched co ntrol subjects from the general population were recruited in Lille and its surroundings in the North of France. Diabetics and subjects takin g hypolipidemic drugs were excluded from the analysis, so that 73 MI a nd 144 control subjects were included. Lipids, apolipoprotein (ape) A- I, apo A-II, apo A-IV and apo B, and apo A-I-containing particles such as lipoproteins containing both apo A-I and apo A-II (LpA-I:A-II) and those containing apo A-I but not apo A-II (LpA-I) were measured in in terstitial fluid by applying mild suction, and in plasma. Univariate a nalysis showed that plasma triglycerides, very low density lipoprotein (VLDL)-cholesterol and apo B were significantly higher, while high de nsity lipoprotein (HDL)-cholesterol, apo A-I, LpA-I and LpA-I:A-II wer e lower in MI survivors compared to controls after adjustment for age, body mass index (BMI), alcohol and tobacco consumption. In interstiti al fluid, cholesterol and apo A-II were higher in MI than in controls before adjustment for covariates. However, after adjustment, triglycer ides became significant while cholesterol and apo A-II remained signif icantly higher in MI, at 43.8 and 7.5 mg/dl, respectively, than in con trol subjects, at 38.6 and 5.9 mg/dl, respectively. Taking into accoun t only the plasma parameters, the multivariate analysis reveals that t riglycerides and apo A-I appear to be independent factors indicative o f the presence of a MI. When plasma and interstitial fluid parameters were taken together in the multivariate analysis, the measurement of a po A-II in interstitial fluid increased the level of prediction of MI over the information provided by the plasma parameters. These data rai se the possibility that interstitial fluid apo A-II levels may be asso ciated with the occurrence of MI.