2-DIMENSIONAL VERSUS 3-DIMENSIONAL CULTURE SYSTEMS - EFFECTS ON GROWTH AND PRODUCTIVITY OF BHK CELLS

The influence of surface growth (two-dimensional microcarriers) and th ree-dimensional growth (aggregates and macroporous supports) in agitat ed, suspended batch culture systems upon growth and productivity of BH K was compared. Cultures using three porous microcarriers (CultiSpher G, Cellsnow EX, and Cytocell), one nonporous microcarrier (Cytodex 3) and natural aggregates were performed in stirred tanks using two diffe rent agitation rates (60 and 100 RPM). With the exception of Cytocell, cell growth, viability, and productivity were similar when three-dime nsional structures (porous microcarriers and aggregates) were used. No nporous microcarriers only compared well at 60 RPM as growth ceased un der overagitation. These results suggest that cultures less susceptibl e to fluid shear are advantageous for scaleup. (C) 1996 John Wiley & S ons, Inc.