A CONVENIENT APPROACH TO THE SYNTHESIS OF TRINUCLEOTIDE PHOSPHORAMIDITES - SYNTHONS FOR THE GENERATION OF OLIGONUCLEOTIDE PEPTIDE LIBRARIES/

Trinucleotide phosphoramidites that correspond to the codons of all 20 amino acids were synthesized in high yield in 5g scale, Precursors of those amidites-trinucleotide phosphotriesters-have been prepared usin g the phosphotriester approach without protection of the 3'-hydroxyl f unction, The structures of trinucleotide phosphotriesters and intermed iates were confirmed by H-1- and P-31-NMR spectra, mass-spectra and by analysis of SPDE-hydrolysates of deprotected preparations, Purity of the target products has been confirmed by test reactions, The synthons have been used for automated synthesis of oligonucleotides and corres ponding libraries by a phosphite-triester approach, A 54mer, containin g 12 randomized internal bases, and a 72mer with 24 internal randomize d bases have been synthesized.