The effect of broad growing conditions in the Pacific Northwest and va
rietal differences on starch damage (191 samples) of soft white winter
(SWW) and club wheat flours were investigated. Starch damage was meas
ured enzymatically by the Megazyme method and iodometrically by a Chop
in Rapid Flour Tester (RFT). The overall average of starch damage (cul
tivars, years, and locations) showed a comparable level in SWW and clu
b wheat flours. The starch damage in club wheat flours was more stable
over crop years than in SWW wheat flours. For individual cultivars, a
veraged over crop years and locations, the SWW cv. Madsen had the high
est percentage of starch damage at 4.77%, while Lewjain and Dusty had
the lowest percentage of starch damage among SWW wheats, at 3.19 and 2
.76%, respectively. Among club wheats, Tres showed the highest percent
age of starch damage at 4.03%, and Moro the lowest with 3.40%. The str
ong correlation between starch damage values measured enzymatically by
the Megazyme method and the electric current reading (E(c)) of the Ch
opin Rapid Flour Tester (RFT) for 191 Buhler milled soft wheat flours
formed the basis for a calibration curve and respective equation. The
equation was introduced into the Chopin RFT instrument and successfull
y used for predicting starch damage in three validation sets of sample
s (41 samples total) with excellent accuracy of 0.29% for soft white w
inter (SWW) wheat flours and 0.10% for club wheat flours. The equation
could to be applicable to unknown commercial blends of soft wheat flo
urs.