DISTRIBUTION OF URIC-ACID IN THE FRACTIONS OBTAINED FROM EXPERIMENTALMILLING OF WHEAT INFESTED WITH GRANARY WEEVIL LARVAE

To determine the late of uric acid during wheat milling, samples of ha rd red winter wheat were inoculated with kernels containing late insta r larvae of the granary weevil (Sitophilus granarius), such that the s amples had a uric acid content of about 25 mu g of uric acid per gram of wheal. The samples were then milled on a Buhler experimental mill t o produce three break flours, three reduction flours, and two millfeed fractions. Uric acid in individual milled fractions was quantified by reversed-phase highperformance liquid chromatography, using ion-pairi ng with tetrabutylammonium phosphate and ultraviolet detection. After milling, over 90% of the uric acid was found in the flour, with approx imately 50% of the total uric acid in the first break fraction. Only a bout 10% of the original uric acid was distributed into the millfeed.