SUBCELLULAR-LOCALIZATION OF ANNEXIN-V IN HUMAN FORESKIN FIBROBLASTS -NUCLEAR-LOCALIZATION DEPENDS ON GROWTH-STATE

Annexin V is a major intracellular calcium-binding protein in human fo reskin fibroblasts. Immunocytochemistry revealed that annexin V was lo calized in the nucleus and throughout the cytoplasm in human foreskin fibroblasts. The presence of annexin V in the nucleus was variable dep ending on the growth state. Nuclear staining was strongest in prolifer ating cells immediately after sub-culture, and decreased on prolonged culture without changing the culture medium. The cytoplasmic location of annexin V was not greatly affected by the same conditions. Refeedin g cells with fresh serum restored annexin V to the nuclei of all cells within in 24 h indicating that nuclear localization of annexin V is d ependent on serum factors.