Jt. Jones et al., PRELIMINARY-X-RAY DIFFRACTION STUDIES OF AN RNA PSEUDOKNOT THAT INHIBITS HIV-1 REVERSE-TRANSCRIPTASE, Acta crystallographica. Section D, Biological crystallography, 52, 1996, pp. 1018-1020
Citations number
14
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Crystals of a 26-nucleotide pseudoknot RNA, PK26, have been grown. The
RNA was produced using phosphoramidite chemistry and was purified by
denaturing polyacrylamide electrophoresis. The crystallization was rob
ust with respect to changes in the number of nucleotides and to the sa
lt used as precipitant. The crystals belong to space group P4(1)22 or
P4(3)22 with unit-cell dimensions a=b=61.6, c=98.9 Angstrom. The best
crystals diffract X-rays to 2.9 Angstrom. Three different sequences in
corporating a single 5-bromo-deoxyuridine or 5-bromo-uridine nucleotid
e were also crystallized. True of these derivatives are being used to
determine the structure by multiple isomorphous replacement.