CRYSTALLIZATION AND PRELIMINARY DIFFRACTION ANALYSIS OF PORCINE HEARTMITOCHONDRIAL NADP(-DEPENDENT ISOCITRATE DEHYDROGENASE())

Authors
ENDRIZZI J ZHANG GY XIONG WFC HURLEY JH REMINGTON SJ COLMAN RF
Citation
J. Endrizzi et al., CRYSTALLIZATION AND PRELIMINARY DIFFRACTION ANALYSIS OF PORCINE HEARTMITOCHONDRIAL NADP(-DEPENDENT ISOCITRATE DEHYDROGENASE()), Acta crystallographica. Section D, Biological crystallography, 52, 1996, pp. 1024-1026
Citations number
22
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
52
Year of publication
1996
Part
5
Pages
1024 - 1026
Database
ISI
SICI code
0907-4449(1996)52:<1024:CAPDAO>2.0.ZU;2-1
Abstract
Isocitrate dehydrogenases [isocitrate:NAD(P)(+) oxidoreductase (decarb oxylating), E.C. 1.1.1.42] are ubiquitous metabolic enzymes which occu r in all living organisms. The NADP(+)-dependent mitochondrial isocitr ate dehydrogenase from pig heart has been crystallized from polyethyle ne glycol/sodium sulfate mixtures in the presence of Mg2+ and isocitra te. The crystals belong to space group C2 with a=137.0, b=113.4, c=65. 0 Angstrom and beta=98.5 degrees, and diffract to at least 2.4 Angstro m resolution. There are two protein monomers per asymmetric unit which are related by non-crystallographic twofold symmetry.