CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF HUMAN DUTPASE

Authors
LARSSON G NYMAN PO SVENSSON LA
Citation
G. Larsson et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF HUMAN DUTPASE, Acta crystallographica. Section D, Biological crystallography, 52, 1996, pp. 1039-1040
Citations number
15
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
52
Year of publication
1996
Part
5
Pages
1039 - 1040
Database
ISI
SICI code
0907-4449(1996)52:<1039:CAPAOH>2.0.ZU;2-9
Abstract
Human dUTPase, expressed in Escherichia coli, has been crystallized. S ingle crystals were obtained by the vapour-diffusion technique using 2 -propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolys is of the substrate. The crystals belong to the orthorombic space grou p P2(1)2(1)2(1) with cell dimensions a=67.51, b=68.26 and c=91.00 Angs trom. The crystallographic asymmetric unit contains one trimer of iden tical subunits.