HYPOOSMOLARITY AND GLUTAMINE INCREASED THE BETA-ACTIN GENE-TRANSCRIPTION IN ISOLATED RAT HEPATOCYTES

The mechanism of action of hydration state was studied on beta-actin g ene expression in isolated hepatocytes, Results obtained with Northern blot analysis and run on transcription assays show that hypoosmolarit y increased and hyperosmolarity decreased the beta-actin mRNA level th rough a corresponding modulation of the rate of the gene transcription , Glutamine, which is known to induce cell swelling, also increased th e beta-actin mRNA level in a dose-dependent manner and induced a stimu lation of the beta-actin gene transcription, Thus, cell hydration stat e regulates gene expression in the liver through a transcriptional mec hanism.