IL-10 INDUCES DNA-BINDING ACTIVITY OF 3 STAT PROTEINS (STAT1, STAT3, AND STAT5) AND THEIR DISTINCT COMBINATORIAL ASSEMBLY IN THE PROMOTERS OF SELECTED GENES

Interaction of IL-10 with its receptor leads to the activation of STAT transcription factors, Herein me report the IL-10 dependent simultane ous activation of three STAT transcription factors: Stat1, Stat3, and Stat5, Upon IL-10 treatment multiple Stat proteins become simultaneous ly activated, and bind to different promoters with equal kinetics but form distinct homo- and heterodimeric transcriptionally active complex es depending on the STAT-consensus elements of a selected gene promote r, Upon IL-10 treatment Stat1, 3, and 5 bind to the GRR of the Fc gamm a RI gene, activated Stat1 and 3 bind to the SIE sequence of the c-fos promoter and transcriptionally active Stat5 assembles at the PRE-STAT consensus sequence of the beta-casein gene, Thus, functionally releva nt STAT dimerization is influenced by the activated cytokine receptor as web as the specific STAT consensus sequence present in a specific g ene promoter.