Spermatozoa from testes and spermatophores of two octopod species, Ele
done cirrhosa and E. moschata, have been investigated by electron micr
oscopy. At the base of the mature sperm acrosome of both species a wel
l developed, periodic, conical structure is present. This structure is
strikingly similar to that present in the Octopus sp. acrosome. Also
the modalities of formation of such a structure during spermiogenesis
show strong similarities between the Octopus and Eledone genera. The r
esistance to disruption of sperm chromatin of E. cirrhosa and E. mosch
ata after treatments with sos and mercaptoethanol which are known to d
issolve disulfide-bridges, reveals the presence of S-S crosslinks.