MOLECULAR-CLONING AND RESTRICTION ENZYME MAPPING OF AVIAN ADENOVIRUS TYPE-8 DNA

Avian adenovirus (AAV) type 8 was cultured in an avian hepatoma cell l ine designated CH-SAH and the viral DNA extracted and purified. Restri ction enzyme analysis of viral DNA using the endonucleases ApaI, EcoRI , HindIII, KpnI, NotI, SpeI, StuI and XbaI was carried out, and fragme nts representing the entire genome were cloned. According to the restr iction enzyme fragments, the size of the AAV type 8 genome was calcula ted to be 44.7 kb. Subcloning of viral DNA fragments and hybridization studies using selected viral DNA fragments facilitated the constructi on of the physical map of AAV type 8 DNA.