OPTIMIZATION OF THE REACTION CONDITIONS FOR THE PEROXYOXALATE CHEMILUMINESCENCE DETECTION SYSTEM OF FLUORESCENT COMPOUNDS IN A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Using an instrument of automatic fluid injection (batch method), we op timized such reaction conditions as pH, concentration and kinds of buf fer for the peroxyoxalate chemiluminscence (PO-CL) detection of fluore scent compounds for high-performance liquid chromatography (HPLC). The results were compared with those obtained by HPLC. Consequently, the usefulness of the batch method for investigating the reaction conditio ns for PO-CL detection in HPLC was demonstrated. With the optimized co ndition using a phosphate buffer for the eluent in HPLC, the detection limits for Dns-Val and dipyridamole were 70 amol and 20 amol on colum n (S/N=2), respectively.