PERITONEAL HEALING AFTER FIBRIN GLUE APPLICATION - A COMPARATIVE-STUDY IN A RAT MODEL

The influence of fibrin glue on adhesion formation and peritoneal heal ing is evaluated in a prospective, randomized, controlled study, In al l, 20 Wistar rats underwent microsurgical suturing of two silicone she ets, one covered with a fibrin glue barrier, to the anterior peritoneu m. Each animal thus served as its own control, After 10 days, adhesion s and peritoneal healing were evaluated by a blinded observer through a second-look laparotomy, Adhesions were scored using a modification o f the classification of Diamond, Tissue around the silicone sheet was examined histologically and by scanning electron microscopy to evaluat e the inflammatory reaction and peritoneal healing (ingrowth of blood vessels and quality of peritoneal cells), Adhesion scores for treated and control sides were (mean +/- SD) 2.89 +/- 4.68 and 6.79 +/- 9.09 ( P = 0.181) respectively, and the percentage of the sheet covered by pe ritoneum was 26.25 +/- 31.50 and 29.21 +/- 40.21 (P = 0.226) respectiv ely, Using the paired Wilcoxon rank test, the P values for the ingrowt h of blood vessels and peritoneal healing evaluated by histology and s canning electron microscopy were 0.842, 0.692 and 0.695 respectively, We conclude that although the mean adhesion score was reduced by >50% by fibrin glue, there is no statistically significant difference conce rning adhesion formation or peritoneal healing with the use of fibrin glue.