EVALUATION OF VIROLOGICAL PROCEDURES TO DETECT FETAL HUMAN CYTOMEGALOVIRUS-INFECTION - AVIDITY OF IGG ANTIBODIES, VIRUS DETECTION IN AMNIOTIC-FLUID AND MATERNAL SERUM

Citation
G. Ruellaneugene et al., EVALUATION OF VIROLOGICAL PROCEDURES TO DETECT FETAL HUMAN CYTOMEGALOVIRUS-INFECTION - AVIDITY OF IGG ANTIBODIES, VIRUS DETECTION IN AMNIOTIC-FLUID AND MATERNAL SERUM, Journal of medical virology, 50(1), 1996, pp. 9-15
Citations number
40
Categorie Soggetti
Virology
Journal title
ISSN journal
01466615
Volume
50
Issue
1
Year of publication
1996
Pages
9 - 15
Database
ISI
SICI code
0146-6615(1996)50:1<9:EOVPTD>2.0.ZU;2-1
Abstract
Human cytomegalovirus (HCMV) is the most common cause of viral intraut erine infection and fetal damage largely due to maternal primary infec tion. Virological procedures which are able to detect HCMV fetal infec tion were evaluated. HCMV IgG antibodies were detected in 62.5% of the pregnant women and 1.47% had a primary infection. From March, 1992 to August, 1995, 29 seroconversions were observed, and in 64 other cases . HCMV IgM antibodies were detected in the first serological test. The mean IgG antibody avidity test (AI) was 31% for the 11 seroconversion s tested and 74% in 32 cases where IgG and IgM HCMV antibodies were de tected in the first serum. In the 29 HCMV seroconversions, 19 amniocen tesis were carried out and 12 fetuses (41.4%) were infected in utero. In four amniotic fluids positive in culture and PCR, the fetus or newb orns were infected and in one out of the two cordocentesis undertaken, hepatitis, anemia, and thrombocytopenia were noted. In four other cas es, investigations seeking HCMV in amniotic fluid were negative wherea s infants were infected at birth. Among the 64 cases with positive HCM V IgM and IgG antibodies detected in the first serological test, three fetuses were infected in utero, but no amniotic fluid was available i n these cases. Amniotic fluids were studied in 39 cases, and HCMV dete ction by culture and PCR-hybridization was negative. HCMV DNA was dete cted in the maternal sera of five out of 21 pairs of seroconversions a nd in two cases on the first negative serum. The assay was also carrie d out on 50 of the 64 HCMV IgM positive sera. Two had detectable HCMV DNA. (C) 1996 Wiley-Liss, Inc.