P. Thylen et al., MONOCYTE AND GRANULOCYTE CD11B CD18, CD62L EXPRESSION AND SICAM-1 CONCENTRATION IN THE INTERDIALYTIC PERIOD/, Nephron, 74(2), 1996, pp. 275-282
We studied cell surface modulation of CD11b/CD18 and CD62L on monocyte
s and granulocytes, sICAM-1 concentrations and the responsiveness of c
ells to exogenous fMLP in patients in the intra- (0-4 h Cuprophan dial
ysis) and interdialytic period (5-28 h) and in healthy subjects (0-24
h). The high CD11b/CD18, low CD62L granulocyte phenotype occurred rapi
dly during dialysis. By contrast, CD62L increased on the subpopulation
of monocytes in circulation initially during dialysis and CD11b/CD18
was mobilized much slower. In the interdialytic period, the CD62L/(CD1
1b/CD18) ratio was reduced up to 12 h after start of treatment on both
monocytes and granulocytes. This ratio was significantly lower than i
n healthy subjects up to 8 h after start of treatment. The responsiven
ess of granulocytes to exogenous fMLP, in terms of CD11b/CD18 mobiliza
tion, was significantly reduced in patients during and after hemodialy
sis as compared to that on granulocytes obtained from healthy controls
. Monocytes were more refractory to fMLP up to 4 h after dialysis. sIC
AM-1 was significantly increased in patients before dialysis as compar
ed to controls and remained elevated and fairly stable throughout trea
tment and in the interdialytic period. The variation in the expression
of adhesion molecules on monocytes and on granulocytes in the interdi
alytic period was not related to the presence of activating serum fact
ors remaining in the circulation after treatment. Our findings emphasi
ze the importance of including the interdialytic period in the evaluat
ion of dialysis membrane biocompatibility, especially when effects on
monocytes are of interest.