SENSITIVE AND UNIVERSAL METHOD FOR MICROBIAL DNA EXTRACTION FROM BLOOD PRODUCTS

Authors
GOLBANG N BURNIE JP KLAPPER PE BOSTOCK A WILLIAMSON P
Citation
N. Golbang et al., SENSITIVE AND UNIVERSAL METHOD FOR MICROBIAL DNA EXTRACTION FROM BLOOD PRODUCTS, Journal of Clinical Pathology, 49(10), 1996, pp. 861-863
Citations number
23
Categorie Soggetti
Pathology
Journal title
Journal of Clinical Pathology → ACNP
ISSN journal
00219746
Volume
49
Issue
10
Year of publication
1996
Pages
861 - 863
Database
ISI
SICI code
0021-9746(1996)49:10<861:SAUMFM>2.0.ZU;2-Z
Abstract
A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichia coli, 80 and 120 cfu for Staphylococcus aureus a nd 20 and 26 cfu for Candida albicans. This compared well with existin g methodologies, worked on limited clinical samples and was not pathog en specific.