SINGLE-STRANDED-DNA OF PLASMID PNB2 IN THERMOPHILIC BACTERIUM CLOSTRIDIUM-THERMOSACCHAROLYTICUM

Authors
DELVER EP BELOGUROVA NG VARFOLOMEEV SD BELOGUROV AA
Citation
Ep. Delver et al., SINGLE-STRANDED-DNA OF PLASMID PNB2 IN THERMOPHILIC BACTERIUM CLOSTRIDIUM-THERMOSACCHAROLYTICUM, Molecular biology, 30(4), 1996, pp. 499-502
Citations number
26
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
30
Issue
4
Year of publication
1996
Part
1
Pages
499 - 502
Database
ISI
SICI code
0026-8933(1996)30:4<499:SOPPIT>2.0.ZU;2-V
Abstract
Plasmid pNB2 (1882 bp, 73% AT), identified earlier in the thermophilic bacterium Clostridium thermosaccharolyticum, was shown to form single -stranded replication intermediates, suggesting that it can replicate via the rolling-circle (RC) mechanism. Analysis of the sequence of the largest open reading frame (867 nt) revealed motifs conserved for Rep proteins that initiate RC replication of plasmids and bacteriophages. The putative pNB2 Rep had highest similarity with those of the pC194/ pUB110 family.