REACTIVITY OF MONOCLONAL-ANTIBODIES OF THE B-CELL PANEL ON PBM FROM BLV-INFECTED AND LYMPHOCYTOTIC COWS

The monoclonal antibodies included in the B cell panel of the Third Wo rkshop on Ruminant Leukocyte Antigens were tested by flow cytometry fo r their reactivity with peripheral blood mononuclear cells (PBM) from normal, BLV (bovine leukemia virus)-infected but non-lymphocytotic and lymphocytotic cows. Three MoAbs probably detected pan-B cell antigens . They detected an increase in the B/T cell ratio in peripheral blood from lymphocytotic cattle, However, no changes were observed in the su rface phenotypes of B cells from infected animals with MoAbs of the wo rkshop panel.