ANALYSIS OF MONOCLONAL-ANTIBODIES REACTIVE WITH MOLECULES UP-REGULATED OR EXPRESSED ONLY ON ACTIVATED LYMPHOCYTES

Monoclonal antibodies potentially specific for antigens expressed or u pregulated on activated leukocytes were selected for further analysis from the panel submitted to the third international workshop on rumina nt leukocyte antigens, The kinetics of expression of these activation antigens on resting peripheral mononuclear cells (PBMC) and PBMC stimu lated with concanavalin A or staphylococcal superantigen SEC1 for 4, 2 4 or 96 h were compared, as well as their appearance on various subset s of cells, For some of them, a molecular mass could be determined aft er immunoprecipitation from radio-labeled, lectin-stimulated cells, Ba sed on the results from the clustering, kinetic studies and biochemica l data, evidence was gathered for assigning two additional mAbs to clu ster BoCD25 (IL-2 receptor) and two mAbs to cluster BoCD71 (transferri n receptor). Four mAbs recognized an early activation antigen predomin antly expressed on gamma delta T cells in short-term cultures, A numbe r of other activation antigens were further characterized.