IDENTIFICATION OF MONOCLONAL-ANTIBODIES WITH SPECIFICITY TO ALPHA-CHAIN OR BETA-CHAIN OF BETA(2)-INTEGRINS USING PERIPHERAL-BLOOD LEUKOCYTES OF NORMAL AND BOVINE LEUKOCYTE ADHESION DEFICIENT (BLAD) CATTLE
Vpmg. Rutten et al., IDENTIFICATION OF MONOCLONAL-ANTIBODIES WITH SPECIFICITY TO ALPHA-CHAIN OR BETA-CHAIN OF BETA(2)-INTEGRINS USING PERIPHERAL-BLOOD LEUKOCYTES OF NORMAL AND BOVINE LEUKOCYTE ADHESION DEFICIENT (BLAD) CATTLE, Veterinary immunology and immunopathology, 52(4), 1996, pp. 341-345
The reactivity of monoclonal antibodies entered in the panels of the T
hird Workshop on Ruminant Leucocyte Antigens with lymphocytes and mono
cytes of normal and beta(2)-integrin deficient (Bovine Leucocyte Adhes
ion Deficiency: BLAD) animals was determined by flow cytometry to inve
stigate potential specificities to alpha- or beta-chains of beta(2)-in
tegrins. From the 13 monoclonal antibodies that were entered as having
specificity for CD11/CD18 antigens, ten were confirmed correct, but t
hree had reactivity with cells from BLAD animals, We conclude that our
approach provides an easy way to reliably identify the majority of be
ta(2)-integrin specific monoclonal antibodies.