IDENTIFICATION OF MONOCLONAL-ANTIBODIES WITH SPECIFICITY TO ALPHA-CHAIN OR BETA-CHAIN OF BETA(2)-INTEGRINS USING PERIPHERAL-BLOOD LEUKOCYTES OF NORMAL AND BOVINE LEUKOCYTE ADHESION DEFICIENT (BLAD) CATTLE

The reactivity of monoclonal antibodies entered in the panels of the T hird Workshop on Ruminant Leucocyte Antigens with lymphocytes and mono cytes of normal and beta(2)-integrin deficient (Bovine Leucocyte Adhes ion Deficiency: BLAD) animals was determined by flow cytometry to inve stigate potential specificities to alpha- or beta-chains of beta(2)-in tegrins. From the 13 monoclonal antibodies that were entered as having specificity for CD11/CD18 antigens, ten were confirmed correct, but t hree had reactivity with cells from BLAD animals, We conclude that our approach provides an easy way to reliably identify the majority of be ta(2)-integrin specific monoclonal antibodies.