IDENTIFICATION OF MONOCLONAL-ANTIBODIES WITH SPECIFICITY TO ALPHA-CHAIN OR BETA-CHAIN OF BETA(2)-INTEGRINS USING PERIPHERAL-BLOOD LEUKOCYTES OF NORMAL AND BOVINE LEUKOCYTE ADHESION DEFICIENT (BLAD) CATTLE

Citation
Vpmg. Rutten et al., IDENTIFICATION OF MONOCLONAL-ANTIBODIES WITH SPECIFICITY TO ALPHA-CHAIN OR BETA-CHAIN OF BETA(2)-INTEGRINS USING PERIPHERAL-BLOOD LEUKOCYTES OF NORMAL AND BOVINE LEUKOCYTE ADHESION DEFICIENT (BLAD) CATTLE, Veterinary immunology and immunopathology, 52(4), 1996, pp. 341-345
Citations number
16
Categorie Soggetti
Immunology,"Veterinary Sciences
ISSN journal
01652427
Volume
52
Issue
4
Year of publication
1996
Pages
341 - 345
Database
ISI
SICI code
0165-2427(1996)52:4<341:IOMWST>2.0.ZU;2-A
Abstract
The reactivity of monoclonal antibodies entered in the panels of the T hird Workshop on Ruminant Leucocyte Antigens with lymphocytes and mono cytes of normal and beta(2)-integrin deficient (Bovine Leucocyte Adhes ion Deficiency: BLAD) animals was determined by flow cytometry to inve stigate potential specificities to alpha- or beta-chains of beta(2)-in tegrins. From the 13 monoclonal antibodies that were entered as having specificity for CD11/CD18 antigens, ten were confirmed correct, but t hree had reactivity with cells from BLAD animals, We conclude that our approach provides an easy way to reliably identify the majority of be ta(2)-integrin specific monoclonal antibodies.