ASSIGNMENT OF GENES-CODING FOR LEUKOCYTE SURFACE MOLECULES TO RIVER BUFFALO CHROMOSOMES

A panel of 35 somatic cell hybrids between river buffalo (Bubalus buba lis L.) and Chinese hamster was used in combination with 40 monoclonal antibodies to find synteny among leukocyte antigens and between alrea dy established synteny groups and leukocyte antigens. Six synteny grou ps were identified and three sets of markers were related to already e xisting synteny groups. Antibody IL-A165 detected CD71, which is synte nic with synteny groups U10 and U25, confirming that U10 and U25 are b oth are on a biarmed river buffalo chromosome. This would imply that U 25 could be assigned to the short arm of the river buffalo chromosome 1 (BBU Ip), as U10 has previously been assigned to this chromosome. An tibodies Buf 32, Buf 24 and BAGB27 detected an antigen syntenic with U 1 and U7, This finding suggests that U1, which is assigned to cattle c hromosome 16, which corresponds to river buffalo chromosome 5q, is syn tenic with U7, and that U7 is located on the short arm of buffalo chro mosome 5. Furthermore, the gene coding for CD14 is assigned to U16, wh ich is on cattle chromosome 11 and expected to be on river buffalo chr omosome 12.