S. Gumuslu et al., THE EFFECTS OF DETERGENTS ON T-BUTYL HYDROPEROXIDE-INDUCED CHEMILUMINESCENCE, International journal of clinical & laboratory research, 26(3), 1996, pp. 203-206
We examined the effects of Triton X-100, digitonin, sodium dodecyl sul
fate, taurocholic acid, and cetylpyridinium chloride on hemoglobin-cat
alyzed and t-butyl hydroperoxide-induced chemiluminescence. The experi
mental system contained hemoglobin, luminol, t-butyl hydroperoxide, an
d different concentrations of detergents (5-100 mg/dl) in TRIS-HCl buf
fer. Control assays were performed by excluding detergents. Chemilumin
escence was detected using a liquid scintillation counter in single ph
oton mode. All concentrations chosen for each detergent reduced the ma
ximum chemiluminescence value and retarded the time that maximum chemi
luminescence occurred. The most prominent reduction in maximum chemilu
minescence was observed with 50 and 100 mg/dl digitonin. The smallest
reduction was observed with 5 mg/dl sodium dodecyl sulfate, without re
tardation of the time that maximum chemiluminescence occurred. Our aim
was to use detergents in membrane-containing experimental systems and
hence to identify the detergent with the least effect on chemilumines
cence. Our results suggest that sodium dodecyl sulfate is the most sui
table detergent for chemiluminescence studies in membrane systems.